A model for the generation of tobacco rattle virus (TRV) anomalous isolates: pea early browning virus RNA-2 acquires TRV sequences from both RNA-1 and RNA-2
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چکیده
منابع مشابه
TRV–GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function
Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy traceable TRV vector, a green fluorescent protein (GFP) gene was tagged to the 3' te...
متن کاملThe complete nucleotide sequence of tobacco rattle virus RNA-1.
The complete nucleotide sequence of tobacco rattle virus (TRV) strain SYM RNA-1 was determined from a series of overlapping cDNA clones. cDNA prepared by primer extension was used to determine the exact 5' terminus. The RNA sequence was 6791 nucleotides in length and contained four open reading frames (ORFs). The ORF nearest the 5' terminus coded for a polypeptide of predicted mol. wt. 134,000 ...
متن کاملRNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene.
The sequence of the 3'-terminal 1210 nucleotides of RNA 1 and the complete sequence of 3389 nucleotides of RNA 2 of tobacco rattle virus (TRV) strain TCM has been deduced. The sequence of the 3'-terminal 1099 nucleotides of RNAs 1 and 2 was found to be identical. Thus the genome of this TRV strain is partially diploid, encoding a 16K protein in both RNA 1 and RNA 2. The sequence that is unique ...
متن کاملTrna-uke Properties of Tobacco Rattle Virus Rna Nucleic Acids
ABBTRACT Thi 3' terainal forty nucleotides of tobraviral RNAs readily fold into a tertiary structure, reseabling that of tyaoand tobaaoviral RNAs. The latttr RNA* possess a tRNA-like structure at their 3' m d that is recognized by a nUBber of tRNA-specific enzyaes (Rietveld et al. (1984), EHBO J. 2, 26132619). Characteristic for thiir aainoacyl acctptor a m is tht priunct of a •o-calltd pseudok...
متن کاملRNA-2 of tobacco rattle virus encodes the determinants of transmissibility by trichodorid vector nematodes.
Pseudorecombinant isolates produced from an efficiently transmitted and a non-transmitted isolate of tobacco rattle tobravirus were tested for their transmissibility by trichodorid vector nematodes. Isolates with RNA-2 originating from the non-transmissible isolate were not transmitted by the vector, whereas isolates with RNA-2 originating from the efficiently transmitted isolate were transmitt...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1991
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-72-7-1751